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Please use this identifier to cite or link to this item:
http://hdl.handle.net/2239/33229
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| Title: | Mycobacterium bovis bacille Calmette-Guerin vaccination of cattle: activation of bovine CD4+ and γdelta TCR+ cells and modulation by 1,25-dihydroxyvitamin D3. |
| Authors: | Waters, W.R.
Nonnecke, B.J.
Foote, M.R.
Maue, A.C.
Rahner, T.E.
Palmer, M.V.
Whipple, D.L.
Horst, R.L.
Estes, D.M.
USDA, ARS |
| Keywords: | T cell receptor-positive T-lymphocytes
T-cell subsets
cattle
Mycobacterium bovis BCG
BCG vaccine
vaccination
immune response
lymphocyte proliferation
T-lymphocytes
CD4-positive T-lymphocytes
receptors
calcitriol
immunomodulators |
| Issue Date: | 17-Oct-2007 |
| Description: | Summary Setting: 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) is a potent modulator of immune responses and may be beneficial in the treatment of tuberculosis. Recent evidence suggest that 1,25(OH)2D3 may affect T-dependent responses in cattle; however, mechanisms by which this vitamin modulates activation of bovine Tcells are unclear. Objective: Determine the effects of 1,25(OH)2D3 on the expression of CD25, CD44, and CD62L by bovine T cell subsets proliferating in response to antigen stimulation. Design: Antigen-specific recall responses of Mycobacterium bovis bacille Calmette-Guerin (BCG) vaccinated cattle were used as a model system to evaluate effects of 1,25(OH)2D3 on the proliferation and activation of bovine T cell subsets. Results: CD4* and γdelta TCR* cells were the predominant T cell subsets responding to soluble crude M. bovis-derived antigens (i.e., purified protein derivative and a BCG whole cell sonicate) by proliferation and activation-induced alterations in phenotype. These subsets exhibited increased CD25 and CD44 mean fluorescence intensity (mfi) and decreased CD62L mfi upon antigen stimulation. Addition of 1,25(OH)2D3 inhibited proliferation of CD4* cells and decreased the expression of CD44 on responding (i.e., proliferating) CD4* and γdelta TCR* cells. Conclusion: These findings suggest that the production of 1,25(OH)2D3 by macrophages within tuberculous lesions would inhibit proliferation and CD44 expression by co-localized CD4* and γdelta TCR* cells. |
| URI: | http://seekspace.resip.ac.cn/handle/2239/33229 |
| Other Identifiers: | http://seekspace.resip.ac.cn/handle/10113/2643 |
| Appears in Collections: | 网络开放学术资源
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